The remains, dating back to the Iron Age, were found at Ferry Fryston three years ago during upgrading of the A1.
The chariot was buried whole in its owner's grave – a fact that makes it a very rare find, say archaeologists.
It forms the centrepiece of an exhibition starting today at Pontefract Museum.
Tests have revealed that the skeleton found with the chariot was that of a male aged 30 to 40, who probably did not originate from the West Yorkshire area.
More than 12,000 fragments of bone from more than 180 cattle were discovered in burial pits around the chariot. At first, these were thought to be the remains of a large sacrificial feast indicating the high social status of the man buried with the chariot. Subsequent tests showed the bones dated from the time of the initial Iron Age burial through to the second century AD, indicating repeated visits to the site over five centuries.
The Wheels of Time exhibition features all the remaining sections of the chariot, plus the skeleton and other artefacts found buried on the site.
Illustrated panels tell the story of the remarkable discovery, give details of the excavation and conservation, and provide an insight
into life in the region at the time.
The Highways Agency, which funded the excavation in 2003, said it was delighted the artefacts were now going on show to the public.
Project manager Alec Briggs said: "This was a unique and intriguing discovery which attracted media attention from around the world.
"We knew from our preliminary investigations there were interesting archaeological features in the area. The discovery of the chariot turned out to be one of the most significant archaeological finds on a road project in the country."
The exhibition runs until July 22. Entry to the museum, in Salter Row in the town centre, is free.