The journalist was previously interviewed under caution at the end of 2013 by officers investigating claims of illegal interception of voicemails by Mirror Group journalists.
A Metropolitan Police spokesman said: “A 50-year-old man was interviewed under caution on April 21 2015 by officers from Operation Golding in connection with suspected conspiracy to intercept telephone voicemails.
“He was interviewed by appointment. He was not arrested.”
The former tabloid boss tweeted: “At 1.30pm today, I left a second police interview re Mirror hacking investigation.
“At 4.30pm, the Guardian phoned me about it.”
In a statement, he said: “Some time ago I was asked to attend an interview with officers from Operation Golding when I was next in the UK.
“This was further to a previous voluntary interview I provided in December 2013. I attended that interview today.
“As this is an ongoing investigation, I am unable to comment further until its conclusion.”