Guiseley newcomer Anthony Dudley did the damage with a fine double against Welling.
It left manager Mark Bower to enthuse: “You can’t ask any more from a debut. It was also a good performance and result from the team.”
Like the first game of the season that Welling won, both sides created plenty of chances, and the visitors were unlucky not to take the lead when Michael Bakare and Barney Williams combined with Rob Atkinson making a good block on the line.
Dudley, who only joined on loan from Bury on Friday, then provided a smart finish after being found in the area by Ryan Toulson.
The hosts nearly doubled their lead when Adam Boyes produced a quick turn and shot but Tom King saved.
Welling then played themselves into a good position with the dangerous Bakare being found in the area but his shot lacked any real power.
Just before half-time, Dudley did it again when he latched onto a pass from Gavin Rothery and converted.
At the start of the second half, Welling almost pulled one back with Afolabi Obafemi heading just wide from a free-kick.
Guiseley could have added to their lead but Boyes had a couple of efforts saved.
But that did not matter for the hosts, who, after their home FA Cup replay defeat to Halifax, got a boost heading into their West Riding County Cup tie v Silsden.
Guiseley: Drench, Toulson, Parker (Porritt 90), Lowe, Lockwood, Lawlor, D. Boshell, Boyes (Craddock 90), Rothery, Atkinson, Dudley. Unused substitutes: Hall, D Atkinson, Marsden.
Welling United: King, Fagan (Jefford 82), Lee, Wellard, Nortey, Obafemi, Taylor (Cumberbatch 71) Lokko, Harris, Williams, Bakare. Unused substitutes: McEntegart, Corne, Vidal.
Referee: S Ross (Lincolnshire).
Man of the match: Anthony Dudley.