Local shoppers descended on the new store at St James Retail Park, with queues outside the shop starting to form at 7.30am - hours ahead of its opening.
A ceremonial ribbon cutting was led by Wilko's Anthony Houghton and Tom Rahilly, a representative from Save the Children who attended on behalf of the Wilko Charity Partnership.
Wilko St James Retail Park branch manager Andrew Billam presented the charity partnership with a £600 cheque for all three charities.
Mr Billam said: “We have been overwhelmed by the support of the local people, it’s a very positive start to our residence here at St James Retail Park and we can’t wait to welcome more customers to the new store.”
The firm remains a third generation, family-owned business after more than 85 years of trading and the chain has 418 stores across the UK.