The blaze took place in Graingers Road, Hornsea, East Yorkshire, a spokeswoman at Humberside Fire and Rescue Service said.
A total of 22 dogs died in the incident which was attended by 18 firefighters. It is understood the dogs were in cages and were owned by private breeders.
It is not known what caused the fire at this stage, the spokeswoman added.
The incident comes after a blaze at the Manchester Dogs Home, just ovea week ago, resulted in at least 50 dogs being killed and others injured.
The fire in Hornsea took place at a wood-structured property which was “totally destroyed” by the fire, a spokeswoman added.
She said firefighters reported it was “well alight” when they got there after being called shortly before 7pm on Saturday evening.
Meanwhile, well-wishers donating to the tragic Manchester Dogs Home saw more than £1.2 million pledged in less than 48 hours after the suspected arson attack on September 11.
Overwhelmed staff were forced to tell people donating supplies to stay away from its sister site in Warrington, Cheshire, after the weight of public benevolence caused tailbacks on the M6 motorway and around Warrington.
A 15-year-old boy arrested on suspicion of starting the blaze has since been released on bail.
After more than 30 firefighters began to tackle the blaze, hundreds of people rallied round to provide refreshments, animal food and other help for the dogs that were rescued.