Police are appealing for any witnesses after the crash at about 11.40am on Saturday.
It occurred on the B6255 at Widdale Foot between Hawes and Newby Head.
A North Yorkshire Police spokesperson said that "a black and red motorcycle left the road and collided with a wall".
The rider, a man in his 30s, suffered serious injuries and was taken to Royal Preston Hospital.
Anyone who saw the motorcycle involved is asked to contact TC 994 Simon Hudson of North Yorkshire Police on 101, or email [email protected], quoting incident number 12170138210.