The women's race kicks off first at 9.05am, followed later by the men at 2.45pm, to cover a distance of 132km (82 miles).
The fifth edition of the now annual race will see riders race their way through 150 villages, towns and cities across Yorkshire, between Thursday 2 and Sunday 5 May.
The men's race will cover a distance of 617.5km (384 miles) over four stages, while the women's race cover 264km (164 miles) over two stages.
Stage 2 - Barnsley to Bedale
The riders will head in a north-easterly direction towards Pontefract, where they will reach the first intermediate sprint of the race (women at 9.45am/men at 3.21pm).
The route then skirts around Leeds before heading to the village of Leathley (10.55am / 4.22pm), and later arriving at the Côte de Lindley (10.58am / 4.25pm) for the first of five new climbs on this year's route.
The peloton will reach Harrogate next (11.07am / 4.32pm), with the second intermediate sprint taking place along Parliament Street (11.21 / 4.45pm).
The route then continues north through Ripon (11.54am / 5.13pm), before heading into Bedale for a sprint finish (12.33pm / 5.47pm).
If you are planning to head out and watch stage 2 of the race, here is a breakdown of all the key timings you need to know:
- 9.05am - Opening of the women's stage at Barnsley Town Hall
- 9.35am - Nostell Priory
- 9.45am - Pontefract - the first intermediate sprint
- 10.11am - Garforth
- 10.23am - Scholes
- 10.58am - Côte de Lindley - first climb
- 11.07am - Harrogate
- 11.21am - Parliament Street - second intermediate sprint
- 11.54am - Ripon
- 12.33pm - Beadle - women's finish
- 2.45pm - Barnsley Town Hall - men's race start
- 3.12pm - Nostell Priory
- 3.21pm - Pontefract - first intermediate sprint
- 3.45pm - Garforth
- 3.53pm - Scholes
- 4.25pm - Côte de Lindley - first climb
- 4.32pm - Harrogate
- 4.45pm - Parliament Street - second intermediate sprint
- 5.13pm - Ripon
- 5.47pm - Beadle - men's finish