Boris Johnson has announced that he will seek to trigger a snap general election after losing a crunch vote on Brexit.
The Prime Minister said he would table a motion under the Fixed-term Parliaments Act on Tuesday night.
But Labour indicated that they would not support the move until chances of a no-deal Brexit were taken off the table.
Mr Johnson told the House of Commons after he was defeated in his first vote: "The consequences of this vote tonight means that Parliament is on the brink of wrecking any deal that we might be able to get in Brussels. It will hand control of the negotiations to the EU."
He continued: "I don't want an election but if MPs vote tomorrow to stop the negotiations and to compel another pointless delay of Brexit, potentially for years, then that will be the only way to resolve this.
"I can confirm that tonight we will are tabling a motion under the Fixed-term Parliament Act."
Moments earlier MPs voted in favour of allowing a cross-party alliance to take control of the Commons agenda on Wednesday in a bid to block a no-deal Brexit on October 31 by 328 votes to 301, majority 27.
Analysis of the Commons division list showed 21 Tories rebelled to support the motion.
Former Cabinet ministers Philip Hammond, Rory Stewart, Ken Clarke, David Gauke and Greg Clark were among the Tories who rebelled in the key vote.
Earlier the Government threatened to withdraw the whip from any Tories who voted against the motion.
Labour leader Jeremy Corbyn said told the Prime Minister: "He wants to table a motion for a general election, fine. Get the Bill through first in order to take no deal off the table."