Supermarket Morrisons is rolling out its same-day online grocery delivery service with Amazon to Sheffield and four other cities across the UK.
The grocery chain said it will expand Morrisons at Amazon to the Steel City as well as Glasgow, Newcastle, Liverpool, Sheffield and Portsmouth over 2019.
The service, which was launched more than three years ago, is already available to Amazon's Prime Now customers in Leeds, Manchester, Birmingham and parts of London.
Morrisons said it will be extended further across the UK in future years.
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The group is also becoming a retailer with Amazon's Prime website and app, and will begin selling directly to customers, through Morrisons at Amazon.
But Morrisons will continue as a wholesaler for all Amazon's other UK grocery offers for customers.
David Potts, chief executive at Morrisons, said: "Morrisons' conveniently located local supermarkets and Amazon's very popular website and customer offer are an ideal combination, offering ultra-fast same-day grocery home delivery for customers in and around cities across Britain."
The home delivery service allows customers to order a full Morrisons shop online, which is then picked up at a local store, and delivered by Amazon.
In some locations, customers can receive deliveries within one hour of the order being placed.
The news comes after Morrisons last month loosened its agreement with Ocado following a catastrophic fire at the online grocer's Andover facility in February.
As well as changes to its warehouse agreements, the exclusivity of the deal with Ocado was also relaxed, potentially allowing Morrisons to agree a new partnership with another digital player.
Under the previous Ocado deal, Morrisons had been prevented from expanding its Amazon venture further.
Doug Gurr, Amazon UK country manager, said Amazon was "committed to growing our grocery business".
"With the Morrisons store on Prime Now, many Prime members can do their full weekly grocery shop online through Prime Now with ultra-fast same day delivery," he added.