Fisher joins left-arm spinner Karl Carver, all-rounder Will Rhodes, pace bowler Josh Shaw and batsman Jonny Tattersall in a 14-strong squad that will be led by Northamptonshire wicketkeeper/batsman Ben Duckett.
Fisher made history when he played for Yorkshire against Leicestershire at Scarborough on June 9.
He took 1-40 from seven overs and scored 10 from four balls in a game Yorkshire lost by three wickets.
David Graveney, the England and Wales Cricket Board’s National Performance Manager, said: “We welcome Matthew Fisher into the squad for the first time along with a number of other new players who have impressed in county second XI and first-team cricket this summer.
“Matthew’s selection reflects both the selectors’ and Yorkshire’s view that he is ready to step up to the challenge of playing international age-group cricket.”
The tri-series starts on August 5 and sees each side play six one-day games before the final at Trent Bridge on August 19.
Squad: Ben Duckett (Northants, capt), Thomas Alsop (Hampshire), Tom Barber (Hampshire), Karl Carver (Yorkshire), Harry Finch (Sussex), Matthew Fisher (Yorkshire), Miles Hammond (Gloucs), Ryan Higgins (Middlesex), Lewis McManus (Hampshire), Will Rhodes (Yorkshire), Josh Shaw (Yorkshire), Dominic Sibley (Surrey), Jonny Tattersall (Yorkshire), Jack Winslade (Surrey).
Australia batsman David Warner failed to press his Ashes claims after he managed just six in his first match since his suspension for punching Yorkshire’s Joe Root in a Birmingham bar.
Warner was this week sent to Australia A’s tour of Africa to gain match practice after he was overlooked for the first Investec Test at Trent Bridge.
The 26-year-old, who has been retained in Australia’s Ashes squad, faced just nine balls against the Zimbabwean Select XI in Harare before he was caught behind off strike bowler Kyle Jarvis.
Australia banned Warner from playing in their two Ashes warm-up matches after the incident with Root, which occurred in the early hours after England’s Champions Trophy win last month.