Strikes from Niall Ennis and Kieran Sadlier plus Jason McCarthy’s own goal earned victory for Doncaster, with former Rovers striker Adebayo Akinfenwa having briefly drawn the visitors level in the 67th minute.
Rovers took the lead in the final minute of the first half when Ennis slotted into the corner.
Wycombe equalised but Rovers were back in front four minutes later as Sadlier pulled away at the back post to sidefoot in a free-kick from Ben Whiteman.
They put the game to bed after 84 minutes when debutant Jason Lokilo curled in a cross which McCarthy turned into his own goal as he looked to clear.
Manager Darren Moore labelled victory as his youthful side’s best of the season.
Moore said: “When I look back on the game, we’ve probably had four players on the pitch under the age of 20 – you’re talking young players against real experience that looked to come and manage the game as they did.
“It’s a massive three points and probably the best for me this season because of who we had on the pitch and what they were up against. I’m absolutely delighted.
“My boys stood up to the experience and know-how that Wycombe had on the pitch. It went to 1-1 and the players showed great resilience and belief to turn it around, go and get the second goal and then get the third. It turned out to be a convincing win but we had to work so hard for it.”
Doncaster Rovers: Dieng, Halliday, Wright, Anderson, Amos, Whiteman, Sadlier, Ramsey (Sheaf 86), Gomes (Blair 81), Ennis (Lokilo 73), Cole. Unused substitutes: Taylor, Jones, Baptiste, Coppinger.
Wycombe Wanderers: Allsop, McCarthy, Stewart, Charles, Jacobson, Gape, Thompson, Smyth (Parker 83), Wheeler, Onyedinma (Freeman 75), Samuel (Akinfenwa 57). Unused substitutes: Jombati, Bloomfield, Nnamdi Ofoborh, Stockdale.
Referee: D Rock (Hertfordshire).