And manager Danny Wilson wasted no time in lining up not one but two replacements – Rangers duo Kyle Hutton and John Fleck.
The Blades had earlier sold former Barnsley striker Daniel Bogdanovic to Blackpool for an undisclosed fee – and rejected an offer from the Seasiders for midfielder Stephen Quinn.
Since suffering relegation to League One at the end of last season, the Blades have allowed a string of high-earning players to leave Bramall Lane.
Bogdanovic, a Maltese international, followed Darius Henderson, Mark Yeates and Jamie Ward out of the exit since the opening of the summer transfer window but the Blades stood firm and would not allow Quinn to go on the cheap.
An impressive start to the season under new manager Danny Wilson has fuelled optimism over an immediate return to the Championship for the Blades.
Quinn, 25, has played an integral role in the first seven games but has made no secret of a desire to play at a higher level.
Sheffield Wednesday manager Gary Megson, meanwhile, spent the majority of the summer voicing concerns about a lack of transfer activity at Hillsborough yet, by last night’s deadline, could have few complaints.
The Owls have signed a total of 10 new players during the window including, over the last 48 hours, Bury striker Ryan Lowe for £100,000 and Slovenian defender David Kasnick.
Both additions will increase the pressure to win promotion but the outlay was offset last night by the departures of defender Mark Beevers to Milton Keynes Dons and midfielder Giles Coke to Bury, both on loan.
Leeds United’s decision to sell winger Max Gradel to French club Saint Etienne injected around £2m into the Elland Road coffers on Tuesday and fans were anxiously awaiting news of reinvestment in the side.
Manager Simon Grayson had been linked with a swoop for Northern Ireland international midfielder Sammy Clingan from Coventry City but the deal failed to materialise.
Doncaster Rovers’ spending plans had to be put on hold when striker Billy Sharp suffered a serious ankle injury on the opening day of the season.
Sharp, who will be sidelined for another three months, wants to move to the Premier League or a club with ambitions to win promotion from the Championship this season.
Rovers accepted a £3m offer from Southampton but Sharp snubbed the opportunity to move to the south coast.
But for the current injury, Sharp would definitely have been among the hottest properties in the market and Rovers would have been looking to spend the proceeds from his sale.
Manager Sean O’Driscoll will, instead, wait for the loan window to re-open and Sharp’s sale could materialise in the New Year.
Hull City manager Nigel Pearson breathed a massive sigh of relief when midfielder Jimmy Bullard finally found a new employer in Ipswich Town earlier this month.
The one-time England international was being paid over £45,000 a week at the KC Stadium and his wages had become a serious drain on Hull’s resources.
Bullard’s departure freed up more money for Pearson who is also reaping the benefits of Assem Allam’s takeover and financial support. Several players dropped down from the Premier League to join the Tigers and Pearson’s latest target was Leicester City striker Martin Waghorn.
The likelihood of that deal going through at the last minute received a significant boost when news came out early in the evening about Leicester City having had their £4m offer accepted by Everton for former Leeds striker Jermaine Beckford.
Beckford’s anticipated arrival at King Power Stadium – should personal terms have been agreed last night – would have opened the door on Waghorn’s move to the Tigers.
At Rotherham United, the sale of Adam Le Fondre had been inevitable since his decision to reject a new deal last season.
New manager Andy Scott was able to plan accordingly, however, bringing in a host of new players to compensate for the striker’s anticipated departure.
The Millers were involved in a total of 22 player moves during the window – showing again the scale of Scott’s rebuilding programme.
Chairman Tony Stewart also maintained his full support for his manager in the transfer market by making the £350,000 from Le Fondre’s sale available.
Scott duly signed striker Alex Revell from Leyton Orient – just days after landing Brett Williams from Reading.
Barnsley have been another of the region’s busiest clubs and, again, that also coincided with the arrival of a new manager determined to stamp his mark quickly on a club.
The £1m sale of captain Jason Shackell to Derby County gave Keith Hill substantial room for manoeuvre in the transfer market along with the departure of a number of other players, including Argentinian midfielder Hugo Colace.
As the Shackell deal was done early in the summer, it also gave Hill a good opportunity to carry out his shopping before the start of the season.
Eight new players arrived at Oakwell prior to the opening game of the season and Hill was also able to top up his squad with the loan capture of Manchester United’s Danny Drinkwater and Middlesbrough’s Cameron Park last week.
Huddersfield Town’s Lee Clark was another manager blessed with the ability to spend big as a result of summer sales.
Town netted over £3m by selling Lee Peltier to Leicester City and Anthony Pilkington to Norwich City with Clark acknowledging that both players had earned the chance to ply their trade at a higher level.
The double deal gave Clark freedom to bring in nine new players, including winger Danny Ward for around £1m from Bolton Wanderers.
Come yesterday’s deadline, it was a case of Town trying to get rid of players as opposed to strengthening. Robbie Simpson, Joey Gudjonsson and Tom Clarke had all been made available, while striker Danny Cadamarteri continued to attract interest.
Bradford City have been the busiest of the Yorkshire clubs during the transfer window although many of their new arrivals are young players who will form part of a ‘development squad’.
The shock departure of manager Peter Jackson last week, so soon after the summer recruitment drive, baffled many supporters.
New manager Phil Parkinson still felt there were gaps to be filled and moved quickly to bring in former Charlton Athletic winger Kyel Reid.
In further moves yesterday, Parkinson signed former Hull City goalkeeper Matt Duke on a two- year deal, Hull winger Jamie Devitt on loan until January, and teenage defender Dean Overson, who had been released by Burnley.
Duke, 34, had been training with Sheffield United since the summer while Devitt, 21, played for Hull in the Carling Cup recently against Macclesfield Town.
Parkinson is still looking to bring in a new striker, but missed out on Charlton Athletic’s Paul Benson.