The Blades had been due to complete their pre-season programme with a friendly at home to Norwich City.
But the newly-promoted Premier League side had to call off matches against Chesterfield and the Blades at short notice this week after positive Covid-19 result within their camp.
Sheffield United are by no means the only side to suffer disruption to their pre-season plans as a result of the virus. Their first-team squad was forced into 10 days of isolation after two players tested positive on the tour of Spain.
But with a new manager in Slavisa Jokanovic, the opportunity for more game-time ahead of their Championship opener at home to Birmingham City would have been welcome. That, rather than Saturday's planned friendly, will now be Jokanovic's first match at Bramall Lane.
The two games he has overseen were friendly wins over Europa Point in Spain, and Wednesday's 4-0 win at Doncaster Rovers.
Lee Bowyer's Birmingham are due to play their fourth pre-season friendly on Saturday.