Forwards Ian Kirke, Jamie Jones-Buchahan and Luke Burgess all limped off in Sunday's win over Bradford Bulls and coach Brian McClennan concedes they have a battle on to be fit for the hugely-anticipated quarter-final at Headingley.
"Hopefully the'll come through but we'll know later in week," he said. "We've had some adversity in our last couple of games where we've not been able to finish with 17 players but we've come through that and we're making progress as a team.
"We're looking forward to Wigan, it'll be a great test and we're not ruling anybody out yet so we'll see how they go."
Leeds are already without the injured Jamie Peacock and Rob Burrow while Brent Webb completes his two-match suspension this weekend as they bid to end their Challenge Cup drought.
The West Yorkshire club have won the Super League title for the last three seasons but have been unable to prosper in the famous knockout competition since 1999.
It is something New Zealander McClennan is looking to put right after recently signing a new contract until the end of next season.
"I'm happy here as long as the club, the players and my family are happy with me and that remains the case," he said.
"The length of the contract is the best way to go for a club as big as Leeds and it also suits me and my family as we are a long way from home.
"There has been a lot of speculation from outside of our organisation about my future but that is not something I've paid any attention too.
"I'm happy here working with a great bunch of people and I'll be trying to make sure 2010 and 2011 are years we will all fondly remember.
"In all honesty, I've not thought much about anything else than getting ready for our game against Wigan on Saturday.
"We know we will have to be at our best because they are the form team in he competition."
BATLEY BULLDOGS have received a boost ahead of their Challenge Cup quarter-fional with Catalans Dragons after the French side last night saw their influential second row Gregory Mounis banned for one match, ruling him out of Saturday's tie.