As well as a place in the hat for Monday’s draw clubs would receive a significant boost to their coffers.
A total of £25,000 awaits those who can successfully negotiate their way past tomorrow’s opponents.
High-flying Harrogate Town will be putting their National League campaign on the back-burner.
They face a stern test when they welcome Wrexham, who are one point better off than Simon Weaver’s side in the league, to Wetherby Road.
Fellow National League side FC Halifax travel to Warrington Town.
Their hosts, who are two rungs below the Shaymen on the footballing ladder, famously reached the second round four years ago.
But Halifax have a strong record in the cup themselves, reaching the competition proper in four of the last five seasons.
The only blot on that record came last season at this stage when they were ousted by Tranmere Rovers.
York City have been unable to manoeuvre themselves past this stage of the competition since dropping out of the Football League two years ago.
They face a trip to fellow National League North side Blyth Spartans, whose name will stoke memories of famous FA Cup runs of yesteryear, most recently when they lost a third round, five-goal thriller to Birmingham City four years ago.
Guiseley, who are just two points behind York in National League North standings, will fancy their chances of reaching the first round proper for a second consecutive campaign.
The Lions, who host seventh-tier side Stourbridge, made history last season by reaching the second round for the first time in the club’s history.
They held Accrington Stanley to a goalless draw at Nethermoor Park in last season’s first round before coming from behind to draw in the replay 1-1.
What followed was a penalty shoot-out victory, thanks in no small part to the goalkeeping of Jonathan Maxted, who saved two spot-kicks and subsequently earned himself a transfer to Accrington, who have since been promoted to League One.
Guiseley fell 3-0 in the second round at Mansfield, but another money-spinning run would certainly not be sniffed at by the Lions.