Cameron Brown and Aron McMahon have set up Turning Point Brew Co in the North Yorkshire town.
The public can try the first beers at an official launch event at The Falcon Tap in Micklegate, York, from this Thursday to Saturday.
The Turning Point premises are on Kirkby Mills Industrial Estate.
The pair said they sought to emulate the success and professionalism of breweries such as Northern Monk in Leeds and Magic Rock in Huddersfield.
Mr McMahon, 39, of Duggleby in the Yorkshire Wolds, had worked for Brass Castle Brewery in Malton then Half Moon Brewery at Ellerton, south east of York.
Mr Brown, 23, who lives in the Bishopthorpe Road area in York, had worked at The Maltings pub in Tanner’s Moat for two years then spent another two years as licensee at The Falcon Tap, while also home-brewing.
The pair met through their work, struck up a friendship, and discovered they had a shared enthusiasm and ambition.
Mr McMahon said: “It’s a turning point for our careers, and we hope when customers discover our beers at the bar it will be a nice turning point in their evening.”
Mr Brown said: “We want to be in the real beer drinkers’ bars that specialise in beer. We really want people to come along to the launch, to meet us and hear about how we got started – and to let us know what they think of the first beers. We’re very proud of what we’ve made so far.”
Two of the first beers will also be on sale at the Reading Beer Festival, which runs from April 27 to 30.