The crash, involving a white Renault Megane and a white off-road motorcycle, happened at around 4.40pm on Cemetery Road, at the junction with Lady Croft Lane, in the Hemingfield area of Barnsley.
The 25-year-old motorcyclist, who was from Barnsley, was left with fatal injuries. His family have been informed and are being looked after by specially-trained police officers.
The driver of the Renault, a 27-year-old man, was not injured.
Witnesses to the collision, or anyone with dashcam footage, should call South Yorkshire Police on 101 quoting incident number 495 of 22 March 2020.