The Harrogate-based brand filmed the Olympic triathletes acting as 'tea couriers' delivering an urgent package to the company's headquarters.
The TV commercial begins with the brothers stepping out of a lake beside the Yorkshire Tea distribution centre before transitioning onto their bikes and cycling between pallets of tea.
They then race each other on foot to the firm's offices - although are told off for running on the factory floor by a safety-conscious foreman.
The advert makes light on the brothers' rivalry at the end of the clip, when a member of staff makes the sportsmen a cuppa each.
Handing a mug over to Olympic champion Alistair, she cheekily says 'oh, it's you who has Gold, isn't it?' - referring to the brand's Gold Blend product as well as his narrow victory over Jonny in Rio.