With their Easter Monday game at Nottingham Forest postponed, the Reds dropped into the bottom three ahead of the lunchtime game with their promotion-chasing rivals.
Just two wins in the last 25 fixtures does not bode well for hopes of survival but Barnsley took plenty of positives from Good Friday’s 2-2 draw against Bristol City.
The Blades are chasing a play-off spot but Moore said: “I can see us turning them over and using that three points for our own benefits. It was annoying and disappointing not to play on Monday as we wanted to carry on our momentum after that performance.
“But we’ll take it into Saturday for the derby. It could be the biggest seven games of our careers. No-one wants to go down and we’ll fight to the end. The mood is good. We’re all upbeat. We know it will be a big ask – it’ll be a fight – but we know we can get points.”
Moore scored the opener against Bristol City, just his third goal in 13 appearances since joining from Ipswich Town in a £750,000 deal at the start of January. He scored 13 times in 22 League One game while on loan at Rotherham United earlier this season and knows his strike-rate will be crucial in the weeks ahead.
The 25-year-old said: “All I think about is scoring. I’m happy to score but also happy to assist others to do so. We made chances against Bristol and it worked well how we played. Going into the derby, it’s just about game-management now and putting those chances away. It’s always a good atmosphere when it comes to South Yorkshire derbies.
“I played in one with Rotherham against Doncaster which was good and I’m looking forward to this now, too.”