Murray decided on Saturday he would play at next week’s Fever-Tree Championships, ending an 11-month absence from the game.
The three-time major winner has not played since last year’s Wimbledon due to a hip injury and subsequent surgery.
He practised at Queen’s on Friday ahead of next week’s Fever-Tree Championships, delayed a decision until Saturday and then told tournament officials he would be there.
“Andy Murray has confirmed that he will play in the Fever-Tree Championships,” the tournament said on its official Twitter account.
Murray played with fellow Brit Cameron Norrie in a relaxed session on Friday but will have a real step up in opposition when he sees Kyrgios over the net.
The Australian is number 24 in the world and is supremely talented, if volatile. Murray, however, has a good record against him, winning all five of their meetings.
Elsewhere in the draw, Novak Djokovic will face a qualifier, top seed Marin Cilic faces Fernando Verdasco and British number one Kyle Edmund will take on Ryan Harrison.