An alert was issued across a wide area warning residents, but there was no reported flooding.
Tests were carried out to ensure the 51-year-old structure was operational ahead of being lowered on Wednesday night for another high tide.
Staff were due to remain at the barrier as a precaution, while residents were advised that Wednesday's tide would be lower and “within the normal range”.
The barrier is operated around 30 times a year, at times when high tides are expected to funnel surges of water into the River Hull. The massive 212-tonne barrier gate can be deployed within 30 minutes of a flood warning.
The expectation is that the barrier - which was put in place following widespread flooding on September 29 1969 - will be used more often in future because of climate change and rising sea levels.