Mowatt, outstanding for the Reds’ in their recent run of nine wins in ten Championship matches which has propelled them into the play-off positions, was dismissed for a second-half tackle on Wycombe’s Scott Kashket by referee Darren Bond.
Barnsley felt that the challenge was not worthy of a red card, with head coach Valerien Ismael particularly incensed afterwards and stating that the club would appeal.
The Football Association have now confirmed that the Reds have won their appeal and Mowatt will not serve a three-match ban and will be available for Saturday's home derby with Sheffield Wednesday.
A club statement read: "Barnsley Football Club can confirm that the appeal against Alex Mowatt’s red card against Wycombe Wanderers has been successful.
"The skipper will now be available for our South Yorkshire derby against Sheffield Wednesday this weekend."
Speaking after the Wycombe game, Ismael commented: “It was never a bad foul – he tackled over the ball without a straight leg and that’s why we can’t accept this and we have to fight against that.”
"Alex knows he didn’t do something wrong. He is frustrated. It was unjust.
"If you remember the game against Bristol (City), there was an attack on Cauley Woodrow which was a clear red card and it was only a yellow card."