The 58-year-old has paid the price for a disappointing campaign, with Spurs seventh in the Premier League and with little chance of making the top four.
That may not prove to be too much of an issue given Tottenham’s involvement in the controversial European Super League - but Mourinho will not be the man to lead them in the new breakaway competition should it materialise.
Mourinho has also been denied the chance of delivering a first piece of silverware to the club in 13 years, with Spurs set to take on Manchester City at Wembley on Sunday.
The former Porto, Chelsea, Inter Milan, Real Madrid and Manchester United manager was appointed in November, 2019, less than 12 hours after Mauricio Pochettino had been dismissed.
A statement from Tottenham read: “The club can today announce that Jose Mourinho and his coaching staff Joao Sacramento, Nuno Santos, Carlos Lalin and Giovanni Cerra have been relieved of their duties.
“Ryan Mason will take first-team training today and a further update will follow in due course.”