The late Beatles star bought the 1956 Austin Princess limousine in August 1971. He used it the following year in a film to promote his album Imagine. The footage featured Lennon alongside his wife Yoko Ono.
The car still features five aeroplane seats that were added by the musician.
The buyer will also receive the original vehicle registration and title document, complete with Lennon’s signature from the original purchase.
The vintage motor was donated to the Austin Rock and Roll Car Museum in Texas in 2008 by previous owner Milton Verret. It is now being sold to to raise money for charity and will be included in an upcoming auction held by RM Sotheby’s .
Some of the proceeds from the sale - at Battersea Evolution, south west London, on September 7 - will be donated to Unicef and Make A Wish America.
The auction house estimates the car will fetch a minimum price of £190,000 and a maximum of £265,000.
A guitar stolen from Lennon in the 1960s sold at auction for £1.6 million last year.
Lennon was a member of band The Beatles, alongside Paul McCartney, Ringo Starr and George Harrison.
He later went on to enjoy a successful solo career before he was murdered in New York in 1980 at the age of 40 by Mark David Chapman.