Holme Valley Mountain Rescue in West Yorkshire were targeted when burglars broke into their base and stole computers and prescription drugs for patients.
The Marsden-based team has been left devastated by the crime, which occurred overnight between Monday and Tuesday this week.
The headquarters on Manchester Road were broken into and a safe was prised from the wall inside the building.
Prescription drugs including Morphine and Diazepam were inside the safe, alongside a laptop and two computer screens.
West Yorkshire Police have confirmed they are investigating the crime and has said a quantity of drugs and paperwork have since been recovered nearby, although much of the safe's contents remains missing.
A statement on the Rescue Service's Facebook page on Wednesday said: "Team members arrived at our HQ in Marsden last night to discover we had been broken into. This has occurred somewhere between 5pm Monday and 6:30pm Tuesday.
"We are working with West Yorkshire Police on their investigations.
"There were a number of items taken which allow us to control a call out smoothly and serve our local community.
"We are insured, and as a team we will not let this stop us helping those in need until the control items are replaced."
A public fundraiser set up has since raised more than £200 for the Rescue Team.